Simple Plex Human CCL18/PARC Cartridge

R&D Systems | Catalog # SPCKB-PS-001408

Simple Plex Human CCL18/PARC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL)

Sensitivity

0.06 pg/mL

Assay Range

0.12-1120 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CCL18/PARC Cartridge Summary

Simple Plex assay for the detection of human CCL18 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CCL18.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 40 41
Mean (pg/mL) 7.63 378 7.07 367
Standard Deviation 0.552 23.0 0.497 41.6
CV% 7.2 6.1 7.0 11.3

Recovery for Simple Plex Human CCL18/PARC Cartridge

Recovery was not necessary as CCL18 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human PARC Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human CCL18/PARC Cartridge

Human PARC Simple Plex Assay Standard Curve

Human PARC Simple Plex Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Alternate Names

AMAC-1, DC-CK1, MIP-4, PARC

Entrez Gene IDs

6362 (Human)

Gene Symbol

CCL18

Additional CCL18/PARC Products

Product Documents for Simple Plex Human CCL18/PARC Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human CCL18/PARC Cartridge

For research use only

Citations for Simple Plex Human CCL18/PARC Cartridge

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