Simple Plex Human CCL18/PARC Cartridge

Simple Plex Cartridge Kit for 72 samples, containing CCL18 for use with Human Cell Culture Supernatant/Plasma/Serum. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system

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Human PARC Simple Plex Assay
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Simple Plex Human CCL18/PARC Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 nL), EDTA Plasma (25 nL), Heparin Plasma (25 nL)
Sensitivity
0.12 pg/mL
Assay Range
0.1 - 1,120 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CCL18.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human CCL18 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assa
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagent

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean 7.63 378 7.07 367
Standard Deviation 0.552 23 0.497 41.6
CV% 7.2 6.1 7 11.3

Recovery

Recovery was not necessary as CCL18 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human PARC Simple Plex Assay Linearity

Data Examples

Human PARC Simple Plex Assay

Human PARC Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Entrez Gene IDs:
6362 (Human)
Alternate Names:
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

FAQs

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