Simple Plex Human CCL19/MIP-3 beta Cartridge
R&D Systems | Catalog # SPCKB-PS-002348
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL19/MIP-3 beta Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one MIP-3 beta assay.
Inter-Assay Precision (Precision between assays) Replicates of each MIP-3 beta control were tested in multiple assays performed by at least three technicians using two lots of reagents for human MIP-3 beta.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 37 | 36 |
| Mean (pg/mL) | 36.4 | 2055 | 34.8 | 1881 |
| Standard Deviation | 1.57 | 115 | 3.51 | 209 |
| CV% | 4.3 | 5.6 | 10.1 | 11.1 |
Recovery for Simple Plex Human CCL19/MIP-3 beta Cartridge
Recovery for the human MIP-3 beta assays was evaluated at three different spiked concentrations of MIP-3 beta protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=3) | 100 | 95-104 |
| Heparin Plasma (n=3) | 97 | 94-100 |
| Serum (n=3) | 101 | 100-103 |
Linearity
Samples containing and/or spiked with high concentrations of human MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL19/MIP-3 beta Cartridge
Human MIP-3 beta Simple Plex Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human MIP-3 beta Assay. Assay range is 1.57-6000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL19/MIP-3 beta
Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).
Alternate Names
Gene Symbol
Additional CCL19/MIP-3 beta Products
Product Documents for Simple Plex Human CCL19/MIP-3 beta Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human CCL19/MIP-3 beta Cartridge
For research use only
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Associated Pathways
