Simple Plex Human CCL20/MIP-3 alpha Cartridge
R&D Systems | Catalog # SPCKB-PS-001170
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL20/MIP-3 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 31 | 28 |
| Mean (pg/mL) | 21.1 | 992 | 19.9 | 918 |
| Standard Deviation | 1.30 | 55.3 | 1.90 | 67.6 |
| CV% | 6.2 | 5.6 | 9.7 | 7.2 |
Recovery for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 100-106 |
| EDTA Plasma (n=4) | 102 | 97-107 |
| Heparin Plasma (n=4) | 100 | 92-111 |
| Serum (n=4) | 101 | 100-103 |
Linearity
Samples containing and/or spiked with high concentrations of human MIP-3 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Human MIP-3 alpha Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL20/MIP-3 alpha
CCL20, also known as MIP-3 alpha, is a chemokine that plays an important role in dendritic cell trafficking and recruitment and activation of T cells. It is upregulated in monocytes, T cells, endothelial cells, epithelial cells, and fibroblasts following inflammatory stimulation. CCL20 signals through CCR6 which is expressed on memory T cells, B cells and bone marrow-derived dendritic cells.
Alternate Names
Gene Symbol
Additional CCL20/MIP-3 alpha Products
Product Documents for Simple Plex Human CCL20/MIP-3 alpha Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human CCL20/MIP-3 alpha Cartridge
For research use only
Related Research Areas
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Associated Pathways
