Simple Plex Human CCL26/Eotaxin-3 Cartridge
R&D Systems | Catalog # SPCKB-PS-000486
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL26/Eotaxin-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Eotaxin-3 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Eotaxin-3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Eotaxin-3.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 38 |
| Mean (pg/mL) | 20.2 | 959 | 21.2 | 952 |
| Standard Deviation | 1.17 | 85.3 | 2.06 | 101 |
| CV% | 5.8 | 8.9 | 9.7 | 10.6 |
Recovery for Simple Plex Human CCL26/Eotaxin-3 Cartridge
Recovery for the human Eotaxin-3 assays was evaluated at three different spiked concentrations of Eotaxin-3 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 108 | 103-113 |
| Heparin Plasma (n=4) | 108 | 99-112 |
| Serum (n=4) | 105 | 85-119 |
Linearity
Samples containing and/or spiked with high concentrations of human Eotaxin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL26/Eotaxin-3 Cartridge
Simple Plex Human Eotaxin-3 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Eotaxin-3 Assay. Assay range is 0.36-3400 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL26/Eotaxin-3
CCL26, also known as Eotaxin-3 and SCYA26, is a small cytokine that is a member of the CC chemokine family. It is only 94 amino acids (aa) in length, with aa 1-23 serving as a putative signal peptide. Human CCL26/Eotaxin-3 has a predicted molecular weight of approximately 11 kDa, and the mature protein only shares 27% aa sequence identity with the mouse ortholog. CCL26/Eotaxin-3 is constitutively expressed at low levels in various tissues including in the heart and ovary. Its expression in upregulated in vascular endothelial cells by IL-13 and IL-4. CCL26/Eotaxin-3 is a selective agonist for CCR3, as serves as a chemoattractant for cells that express CCR3, such as eosinophils, basophils, and Th2 cells. It has also been shown to act as an antagonist for the receptors CCR1 and CCR5. CCL26/Eotaxin-3 plays a role in allergic reactions.
Additional CCL26/Eotaxin-3 Products
Product Documents for Simple Plex Human CCL26/Eotaxin-3 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human CCL26/Eotaxin-3 Cartridge
For research use only
Related Research Areas
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Associated Pathways
