Simple Plex Human CCL26/Eotaxin-3 Cartridge

Catalog #: SPCKB-PS-000486 Datasheet / COA / SDS

Simple Plex Human CCL26/Eotaxin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD28, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000486
SPCKA-PS-000486
SPCKC-PS-000486

Control Products Available

898540: Simple Plex Control for Human CCL26/Eotaxin-3

Ella Automated ELISA Platform and Simple Plex Human Eotaxin-3 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Related Research Areas

Simple Plex Human CCL26/Eotaxin-3 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.17 pg/mL

Assay Range

0.4 - 3,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human Eotaxin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human Eotaxin-3 in serum and plasma (EDTA/Heparin) in Sample Diluent SD28.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Eotaxin-3 assay

Inter-Assay Precision (Precision between assays) Replicates of each Eotaxin-3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Eotaxin-3

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	38	38
Mean (pg/mL)	20.2	959	21.2	952
Standard Deviation	1.17	85.3	2.06	101
CV%	5.8	8.9	9.7	10.6

Recovery

Recovery for the human Eotaxin-3 assays was evaluated at three different spiked concentrations of Eotaxin-3 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	108	103-113
Heparin Plasma (n=4)	108	99-112
Serum (n=4)	105	85-119

Linearity

Samples containing and/or spiked with high concentrations of human Eotaxin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human Eotaxin-3 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Eotaxin-3 Assay. Assay range is 0.36-3400 pg/mL.