Simple Plex Human CCL26/Eotaxin-3 Cartridge

Catalog #: SPCKB-PS-000486 Datasheet / COA / SDS
Simple Plex Human CCL26/Eotaxin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD28, and wash buffer
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Control Products Available
Human Eotaxin-3 Simple Plex Assay
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Product Details
Supplemental Products

Simple Plex Human CCL26/Eotaxin-3 Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
0.36 pg/mL
Assay Range
0.4 - 3,400 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant human Eotaxin-3.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Eotaxin-3 in serum and plasma (EDTA/Heparin).


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 20.2 959 21.2 952
Standard Deviation 1.17 85.3 2.06 101
CV% 5.8 8.9 9.7 10.6


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 108 103-113
Heparin Plasma (n=4) 108 99-112
Serum (n=4) 105 85-119


Samples containing and/or spiked with high concentrations of human Eotaxin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Eotaxin-3 Simple Plex Assay Linearity

Scientific Data

Human Eotaxin-3 Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


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