Simple Plex Human CCL7/MCP-3 Cartridge
R&D Systems | Catalog # SPCKB-PS-000477
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL7/MCP-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 13 | 13 |
| Mean (pg/mL) | 9.31 | 492 | 9.18 | 47.4 |
| Standard Deviation | 0.482 | 30.6 | 0.625 | 43.4 |
| CV% | 5.2 | 6.2 | 6.8 | 9.2 |
Recovery for Simple Plex Human CCL7/MCP-3 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 116 | 111-121 |
| EDTA Plasma (n=4) | 99 | 93-105 |
| Heparin Plasma (n=4) | 90 | 85-96 |
| Serum (n=4) | 92 | 81-105 |
Linearity
Samples containing and/or spiked with high concentrations of human MCP-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL7/MCP-3 Cartridge
Human CCL7/MCP-3 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL7/MCP-3/MARC
Human MCP-3 and mouse MARC are members of the CC subfamily of chemokines and are monocyte chemoattractants. Sequence comparisons suggest that MARC may be the mouse homolog of the human MCP-3 gene. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene.
Additional CCL7/MCP-3/MARC Products
Product Documents for Simple Plex Human CCL7/MCP-3 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human CCL7/MCP-3 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human CCL7/MCP-3 Cartridge
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Associated Pathways
