Simple Plex Human CCL8/MCP-2 Cartridge
R&D Systems | Catalog # SPCKB-PS-000478
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL8/MCP-2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 33 | 33 |
| Mean (pg/mL) | 5.15 | 201 | 5.30 | 227 |
| Standard Deviation | 0.266 | 16.6 | 0.598 | 16.5 |
| CV% | 5.2 | 8.3 | 11.3 | 7.3 |
Recovery for Simple Plex Human CCL8/MCP-2 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 97 | 93-102 |
| Heparin Plasma (n=4) | 97 | 86-103 |
| Serum (n=4) | 101 | 92-107 |
Linearity
Samples containing and/or spiked with high concentrations of human MCP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL8/MCP-2 Cartridge
Human MCP-2 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL8/MCP-2
MCP-2 is a CC chemokine subfamily monocyte chemotactic protein. MCP-2 also chemoattracts activated NK cells, CD4+ and CD8+ T lymphocytes, and eosinophils and can induce histamine secretion from basophils. MCP-2 shares 62% amino acid sequence identity with MCP-1 and 58% with MCP-3.
Additional CCL8/MCP-2 Products
Product Documents for Simple Plex Human CCL8/MCP-2 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human CCL8/MCP-2 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human CCL8/MCP-2 Cartridge
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Associated Pathways
