Simple Plex Human CD27/TNFRSF7 Cartridge

Catalog #: SPCKB-PS-000639 Datasheet / COA / SDS

Simple Plex Human CD27/TNFRSF7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000639
SPCKA-PS-000639
SPCKC-PS-000639

Control Products Available

898284: Simple Plex Control for Human CD27/TNFRSF7

Ella Automated ELISA Platform and Simple Plex Human CD27 Assay

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human CD27/TNFRSF7 Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL), Cerebrospinal Fluid (13 uL)

Sensitivity

30.7 pg/mL

Assay Range

30.7 - 46,900 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Specificity

Natural and recombinant human CD27.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay is for the detection of human CD27 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD27 assay

Inter-Assay Precision (Precision between assays) Replicates of each CD27 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD27

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (pg/mL)	346	14787	310	14320
Standard Deviation	15.2	1440	20.7	960
CV%	4.4	9.7	6.7	6.7

Recovery

Recovery for the human CD27 assays was evaluated at three different spiked concentrations of CD27 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=7)	102	94-117

Linearity

Samples containing and/or spiked with high concentrations of human CD27 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Simple Plex Human CD27 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD27 Assay. Assay range is 30.7-46,900 pg/mL.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD27/TNFRSF7

Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is induced by antigen-receptor activation in B cells.

Entrez Gene IDs:

939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)

Alternate Names:

CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7