Simple Plex Human CX3CL1/Fractalkine Cartridge

R&D Systems | Catalog # SPCKB-PS-000488

Simple Plex Human CX3CL1/Fractalkine assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

18.2 pg/mL

Assay Range

56.8-86710 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Cerebrospinal Fluid)

Simple Plex Human CX3CL1/Fractalkine Cartridge Summary

Simple Plex assay for the detection of human Fractalkine in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CX3CL1/Fractalkine Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Fractalkine.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 12 12
Mean (pg/mL) 504 26912 499 25992
Standard Deviation 23.1 1598 26.0 1434
CV% 4.6 5.9 5.2 5.5

Recovery for Simple Plex Human CX3CL1/Fractalkine Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 110 102-127
EDTA Plasma (n=4) 101 93-112
Heparin Plasma (n=4) 99 93-113
Serum (n=4) 112 103-121
Urine (n=6) 92 77-103

Linearity

Samples containing and/or spiked with high concentrations of human Fractalkine were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Fractalkine Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human CX3CL1/Fractalkine Cartridge

Human Fractalkine Simple Plex Assay Standard Curve

Human Fractalkine Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CX3CL1/Fractalkine

CX3CL1/Fractalkine is a transmembrane adhesion protein with a chemokine domain separated from the membrane by a mucin stalk. It is upregulated on many cell types during inflammation. The chemokine and mucin regions can be shed as a soluble chemokine that signals through the receptor CX3CR1. During extravasation, membrane-bound CX3CL1 traps leukocytes, then is cleaved to allow diapedesis. Soluble CX3CL1 protects neurons from microglial neurotoxicity, recruits macrophages for wound healing, recruits osteoclast precursors for bone resorption, and contributes to pathogenesis in coronary artery disease.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

Additional CX3CL1/Fractalkine Products

Product Documents for Simple Plex Human CX3CL1/Fractalkine Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CX3CL1/Fractalkine Cartridge

For research use only

Citations for Simple Plex Human CX3CL1/Fractalkine Cartridge

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