Simple Plex Human CXCL1/GRO alpha Cartridge

R&D Systems | Catalog # SPCKB-PS-012007

Simple Plex Human CXCL1/GRO alpha assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD13, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), Platelet-poor EDTA Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL), Saliva (25 µL), Urine (25 µL)

Sensitivity

0.18 pg/mL

Assay Range

1.15-1760 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Urine)

Simple Plex Human CXCL1/GRO alpha Cartridge Summary

Simple Plex assay for the detection of human CXCL1/GRO alpha (GRO alpha ) in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), urine, and saliva in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CXCL1/GRO alpha/KC/CINC-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human GRO alpha. 

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 50 46
Mean (pg/mL) 24.7 1155 14.6 761
Standard Deviation 1.43 74.8 1.26 52.0
CV% 5.8 6.5 8.6 6.8

Recovery for Simple Plex Human CXCL1/GRO alpha Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 99 99-99
Platelet-poor EDTA Plasma (n=4) 92 88-94
Urine (n=4) 97 87-104

Linearity

Samples containing and/or spiked with high concentrations of human GRO alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human GRO alpha Ella Assay

Scientific Data Images for Simple Plex Human CXCL1/GRO alpha Cartridge

Simple Plex Human GROa Ella Assay

Simple Plex Human GRO alpha Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL1/GRO alpha/KC/CINC-1

CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes,  mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.

Alternate Names

CINC-1, CINC1, GRO alpha, KC, MGSA-alpha

Entrez Gene IDs

2919 (Human); 14825 (Mouse); 81503 (Rat)

Gene Symbol

CXCL1

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Product Documents for Simple Plex Human CXCL1/GRO alpha Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CXCL1/GRO alpha Cartridge

For research use only

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