Simple Plex Human CXCL11/I-TAC Cartridge

R&D Systems | Catalog # SPCKB-PS-000369

Simple Plex Human CXCL11/I-TAC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD16, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.181 pg/mL

Assay Range

0.545-2080 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CXCL11/I-TAC Cartridge Summary

Simple Plex assay is for the detection of human Interferon-inducible T cell alpha Chemoattractant (I-TAC) serum and plasma (EDTA/Heparin) in Sample Diluent SD16. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human I-TAC.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one I-TAC assay.

Inter-Assay Precision (Precision between assays) Replicates of each I-TAC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human I-TAC.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 8 5
Mean (pg/mL) 22.0 1212 21.9 1164
Standard Deviation 0.809 83.9 1.31 173
CV% 3.7 6.9 6.0 14.9

Recovery for Simple Plex Human CXCL11/I-TAC Cartridge

Recovery was not necessary as human I-TAC shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human I-TAC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human I-TAC Assay Linearity

Scientific Data Images for Simple Plex Human CXCL11/I-TAC Cartridge

Simple Plex Human I-TAC Assay Standard Curve

Simple Plex Human I-TAC Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human I-TAC Assay. Assay range is 0.545-2080 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

Additional CXCL11/I-TAC Products

Product Documents for Simple Plex Human CXCL11/I-TAC Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human CXCL11/I-TAC Cartridge

For research use only

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