Simple Plex Human CXCL12/SDF-1 alpha Cartridge
Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Product Summary
Precision
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 38 | 41 |
| Mean (pg/mL) | 27.1 | 1221 | 22.7 | 1049 |
| Standard Deviation | 1.56 | 66.5 | 3.05 | 77.4 |
| CV% | 5.8 | 5.4 | 13.4 | 7.4 |
Recovery
Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: CXCL12/SDF-1 alpha
CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.
FAQs
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