Simple Plex Human CXCL12/SDF-1 alpha Cartridge

Catalog #: SPCKB-PS-000299 Datasheet / COA / SDS
Simple Plex Human CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer
Catalog # Availability Size / Price Qty
Control Products Available
Human SDF-1 alpha  Ella Assay
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Product Details
Supplemental Products

Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary

Sample Type & Volume Required Per Well*
Platelet-poor EDTA Plasma (5 uL), Platelet-poor Heparin Plasma (5 uL)
6.57 pg/mL
Assay Range
6.0 - 1,476 pg/mL (Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Natural and recombinant human SDF-1 alpha.
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human SDF-1 alpha in platelet-poor plasma (EDTA/Heparin)


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 38 41
Mean (pg/mL) 27.1 1221 22.7 1049
Standard Deviation 1.56 66.5 3.05 77.4
CV% 5.8 5.4 13.4 7.4


Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.


Samples containing and/or spiked with high concentrations of human SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human SDF-1 alpha  Ella Assay Linearity

Scientific Data

Human SDF-1 alpha Ella Assay Standard Curve

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.

Entrez Gene IDs:
6387 (Human); 20315 (Mouse); 493806 (Feline)
Alternate Names:
CXCL12 chemokine (C-X-C motif) ligand 12; CXCL12/SDF-1 alpha; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1


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