Simple Plex Human CXCL12/SDF-1 alpha Cartridge

R&D Systems | Catalog # SPCKB-PS-000299

Simple Plex Human CXCL12/SDF-1 alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Platelet-poor EDTA Plasma (5 µL), Platelet-poor Heparin Plasma (5 µL)

Sensitivity

6.57 pg/mL

Assay Range

6.04-1476 pg/mL (Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)

Simple Plex Human CXCL12/SDF-1 alpha Cartridge Summary

Simple Plex assay for the detection of human SDF-1 alpha in platelet-poor plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human SDF-1 alpha.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 38 41
Mean (pg/mL) 27.1 1221 22.7 1049
Standard Deviation 1.56 66.5 3.05 77.4
CV% 5.8 5.4 13.4 7.4

Recovery for Simple Plex Human CXCL12/SDF-1 alpha Cartridge

Recovery was not necessary as SDF-1 alpha shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human SDF-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human SDF-1 alpha  Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human CXCL12/SDF-1 alpha Cartridge

Human SDF-1 alpha  Simple Plex Assay Standard Curve

Human SDF-1 alpha Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.

 

Alternate Names

SDF1 alpha, SDF1a

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline)

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 alpha Products

Product Documents for Simple Plex Human CXCL12/SDF-1 alpha Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CXCL12/SDF-1 alpha Cartridge

For research use only

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