Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

R&D Systems | Catalog # SPCKB-PS-000375

Simple Plex Human CXCL13/BLC/BCA-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.37 pg/mL

Assay Range

1.6-6110 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CXCL13/BLC/BCA-1 Cartridge Summary

Simple Plex assay is for the detection of human B-Lymphocyte Chemoattractant (BLC/BCA-1) in serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CXCL13/BLC/BCA-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human BLC/BCA-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BLC/BCA-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each BLC/BCA-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BLC/BCA-1.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 14 13
Mean (pg/mL) 41.0 1797 38.6 1772
Standard Deviation 1.40 102 2.41 85.6
CV% 3.4 5.7 6.2 4.8

Recovery for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Recovery for the human BLC/BCA-1 assays was evaluated at three different spiked concentrations of BLC/BCA-1 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 83 76-87
Heparin Plasma (n=4) 78 76-83
Serum (n=4) 81 77-84

Linearity

Samples containing and/or spiked with high concentrations of human BLC/BCA-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human BLC/BCA-1 Assay Linearity

Scientific Data Images for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Simple Plex Human BLC/BCA-1 Assay Standard Curve

Simple Plex Human BLC/BCA-1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BLC/BCA-1 Assay. Assay range is 1.6-6110 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL13/BLC/BCA-1

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell  homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naive B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer's patch development.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

Additional CXCL13/BLC/BCA-1 Products

Product Documents for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

For research use only

Citations for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

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