Simple Plex Human CXCL5/ENA-78 Cartridge
R&D Systems | Catalog # SPCKB-PS-000334
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CXCL5/ENA-78 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 32.6 | 1511 | 31.2 | 1446 |
| Standard Deviation | 2.14 | 117 | 1.33 | 73.3 |
| CV% | 6.5 | 7.8 | 4.3 | 5.1 |
Recovery for Simple Plex Human CXCL5/ENA-78 Cartridge
Recovery was not necessary as ENA-78 shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human ENA-78 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CXCL5/ENA-78 Cartridge
Human ENA-78 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CXCL5/ENA-78
CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.
Additional CXCL5/ENA-78 Products
Product Documents for Simple Plex Human CXCL5/ENA-78 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human CXCL5/ENA-78 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human CXCL5/ENA-78 Cartridge
View all citations for the Simple Plex platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Powered by Bioz
Customer Reviews for Simple Plex Human CXCL5/ENA-78 Cartridge
There are currently no reviews for this product. Be the first to review Simple Plex Human CXCL5/ENA-78 Cartridge and earn rewards!
Have you used Simple Plex Human CXCL5/ENA-78 Cartridge?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Associated Pathways
