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Simple Plex Human CXCL9/MIG Cartridge

R&D Systems | Catalog # SPCKB-PS-000402

Simple Plex Human CXCL9/MIG assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with PS (Plasma/Serum).
R&D Systems

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.


Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

3.8 pg/mL

Assay Range

19.9-30400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human CXCL9/MIG Cartridge Summary

Simple Plex assay for the detection of human MIG in cell culture supernatan (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human MIG.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 19 19
Mean (pg/mL) 185 9368 178 9705
Standard Deviation 15.1 765 18.1 1072
CV% 8.2 8.2 10.2 11.1

Recovery for Simple Plex Human CXCL9/MIG Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 100 94-104
Heparin Plasma (n=4) 100 89-109
Serum (n=4) 102 93-111

Linearity

Samples containing and/or spiked with high concentrations of human MIG were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human MIG Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human CXCL9/MIG Cartridge

Human MIG Simple Plex Assay Standard Curve

Human MIG Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Alternate Names

MIG

Entrez Gene IDs

4283 (Human); 17329 (Mouse); 246759 (Rat)

Gene Symbol

CXCL9

Additional CXCL9/MIG Products

Product Documents for Simple Plex Human CXCL9/MIG Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human CXCL9/MIG Cartridge

For research use only

Citations for Simple Plex Human CXCL9/MIG Cartridge

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