Simple Plex Human E-Cadherin Cartridge

R&D Systems | Catalog # SPCKB-PS-000208

Simple Plex Human E-Cadherin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

13.3 pg/mL

Assay Range

9.92-15140 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human E-Cadherin Cartridge Summary

Simple Plex assay is for the detection of human E-Cadherin in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all E-Cadherin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human E-Cadherin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one E-Cadherin assay.

Inter-Assay Precision (Precision between assays) Replicates of each E-Cadherin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human E-Cadherin.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 23
Mean (pg/mL) 75.0 3596 67.7 3841
Standard Deviation 1.79 134 6.83 399
CV% 2.4 3.7 10.1 10.4

Recovery for Simple Plex Human E-Cadherin Cartridge

Recovery for the human E-Cadherin assays was evaluated at three different spiked concentrations of E-Cadherin protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 100 94-107

Linearity

Samples containing and/or spiked with high concentrations of human E-Cadherin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human E-Cadherin Assay Linearity

Scientific Data Images for Simple Plex Human E-Cadherin Cartridge

Simple Plex Human E-Cadherin Assay Standard Curve

Simple Plex Human E-Cadherin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human E-Cadherin Assay. Assay range is 9.92-15140 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: E-Cadherin

E-Cadherin/Cadherin-1 (ECAD) Is a transmembrane cell adhesion protein. It forms homophilic clusters between epithelial cells and is a critical component of adherens junctions. These junctions are critical for the maintenance of the epithelial barrier and epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition (EMT) and cell migration, and E-Cadherin loss contributes to cancer metastasis. The shed ECD is released as a soluble factor that enhances cancer cell motility and EGFR-dependent survival and proliferation.

Alternate Names

Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Gene Symbol

CDH1

Additional E-Cadherin Products

Product Documents for Simple Plex Human E-Cadherin Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human E-Cadherin Cartridge

For research use only

Citations for Simple Plex Human E-Cadherin Cartridge

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail