Simple Plex Human Endothelin-1 Cartridge

R&D Systems | Catalog # SPCKB-PS-000265

Simple Plex Human Endothelin-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.11 pg/mL

Assay Range

0.26-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Endothelin-1 Cartridge Summary

Simple Plex assay for the detection of human Endothelin-1 in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Endothelin-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Endothelin-1.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 9 8
Mean (pg/mL) 6.66 277 6.71 289
Standard Deviation 0.287 8.22 0.426 26.1
CV% 4.3 3.0 6.3 9.0

Recovery for Simple Plex Human Endothelin-1 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 90-102
Heparin Plasma (n=4) 91 81-98
Serum (n=4) 85 82-88

Linearity

Samples containing and/or spiked with high concentrations of human Endothelin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Endothelin-1 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human Endothelin-1 Cartridge

Human Endothelin-1 Simple Plex Assay Standard Curve

Human Endothelin-1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1) is a vascular endothelium derived peptide that acts as a potent vasoconstrictor. It is processed by a furin-like protease to yield a peptide termed Big ET-1. Big ET-1 is then cleaved by ECE-1, producing the mature form ET-1 (1-21). ET-1 expression is induced by mechanical stimulation, various hormones, and pro-inflammatory cytokines. Its production is inhibited by nitric oxide, prostacyclin, and atrial natriuretic peptide (ANP). ET-1 exerts its vasoconstrictor effects primarily through the receptor ETA on vascular smooth muscle cells. It also acts through the ETB receptor on vascular smooth muscle and endothelial cells. ET-1 additionally stimulates cardiac myocyte proliferation and contractility, regulates secretion by hypothalamic and pituitary cells, and promotes neutrophil extravasation and activation.

Alternate Names

ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Entrez Gene IDs

1906 (Human); 13614 (Mouse); 24323 (Rat)

Gene Symbol

EDN1

Additional Endothelin-1 Products

Product Documents for Simple Plex Human Endothelin-1 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Endothelin-1 Cartridge

For research use only

Citations for Simple Plex Human Endothelin-1 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Endothelin-1 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Endothelin-1 Cartridge and earn rewards!

Have you used Simple Plex Human Endothelin-1 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card