Simple Plex Human Endothelin-1 Cartridge

Simple Plex Cartridge Kit for quantitation of Endothelin-1. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
SPCKB-PS-000265
SPCKA-PS-000265
SPCKC-PS-000265
Control Products Available
Human Endothelin-1 Simple Plex Assay
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Simple Plex Human Endothelin-1 Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.1 pg/mL
Assay Range
0.1 - 1,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Endothelin-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Endothelin-1 in serum and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 6.66 277 6.71 289
Standard Deviation 0.287 8.22 0.426 26.1
CV% 4.3 3 6.3 9

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 90-102
Heparin Plasma (n=4) 91 81-98
Serum (n=4) 85 82-88

Linearity

Samples containing and/or spiked with high concentrations of human Endothelin-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Endothelin-1 Simple Plex Assay Linearity

Data Examples

Human Endothelin-1 Simple Plex Assay Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.

To view our complete solutions for Endothelin research, visit bio-techne.com.

Entrez Gene IDs:
1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names:
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

FAQs

No product specific FAQs exist for this product, however you may

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