Simple Plex Human ENPP-2/Autotaxin Cartridge
R&D Systems | Catalog # SPCKB-PS-001780
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human ENPP-2/Autotaxin Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ENPP-2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each ENPP-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ENPP-2.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 32 | 32 |
| Mean (pg/mL) | 723 | 41,109 | 766 | 36,307 |
| Standard Deviation | 33.1 | 2677 | 77.8 | 3520 |
| CV% | 4.6 | 6.5 | 10.2 | 9.7 |
Recovery for Simple Plex Human ENPP-2/Autotaxin Cartridge
Recovery was not necessary as human ENPP-2 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human ENPP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human ENPP-2/Autotaxin Cartridge
Simple Plex Human ENPP-2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human ENPP-2 Assay. Assay range is 31.8-121,440 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: ENPP-2/Autotaxin
ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. Some NPPs hydrolyze phosphates from nucleotides and their derivatives. ENPP-2 shares 40 - 50% identity to ENPP1 & 3, all of which contain a N-terminal intracellular domain, a single transmembrane domain and a large extracellular domain that includes a catalytic domain, two somatomedin-B-like domains, and a C-terminal nuclease-like domain. Unlike ENPP-1 and ENPP-3, ENPP-2 has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. The hydrolysis of nucleotides and lysophospholipids by ENPP-2 is mediated by a single catalytic site. Evidence shows LPA and sphingosine 1-phosphate to be specific inhibitors of ENPP-2. ENPP-2 was originally found to stimulate tumor cell motility and has since been found to enhance tumor invasion and metastasis and to be up-regulated in several types of carcinomas including breast and lung.
Long Name
Alternate Names
Gene Symbol
Additional ENPP-2/Autotaxin Products
Product Documents for Simple Plex Human ENPP-2/Autotaxin Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human ENPP-2/Autotaxin Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human ENPP-2/Autotaxin Cartridge
View all citations for the Simple Plex platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Powered by Bioz
Customer Reviews for Simple Plex Human ENPP-2/Autotaxin Cartridge
There are currently no reviews for this product. Be the first to review Simple Plex Human ENPP-2/Autotaxin Cartridge and earn rewards!
Have you used Simple Plex Human ENPP-2/Autotaxin Cartridge?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review