Simple Plex Human ErbB2/Her2 Cartridge

R&D Systems | Catalog # SPCKB-PS-000335

Simple Plex Human ErbB2/Her2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL)

Sensitivity

2.54 pg/mL

Assay Range

13.1-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human ErbB2/Her2 Cartridge Summary

Simple Plex assay is for the detection of human ErbB2 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human ErbB2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ErbB2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each ErbB2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ErbB2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 15 35
Mean (pg/mL) 48.0 2043 52.3 2223
Standard Deviation 2.88 141 3.14 172
CV% 6.0 6.9 6.0 7.8

Recovery for Simple Plex Human ErbB2/Her2 Cartridge

Recovery for the human ErbB2 assays was evaluated at three different spiked concentrations of ErbB2 protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 108 106-111
Urine (n=4) 112 97-125

Linearity

Samples containing and/or spiked with high concentrations of human ErbB2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human ErbB2 Assay Linearity

Scientific Data Images for Simple Plex Human ErbB2/Her2 Cartridge

Simple Plex Human ErbB2 Assay Standard Curve

Simple Plex Human ErbB2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human ErbB2 Assay. Assay range is 13.1-8000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for Simple Plex Human ErbB2/Her2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human ErbB2/Her2 Cartridge

For research use only

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