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Simple Plex Human Erythropoietin Cartridge

R&D Systems | Catalog # SPCKB-PS-000487

Simple Plex Human Erythropoietin/EPO assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.202 mIU/mL

Assay Range

0.36-550 mIU/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Erythropoietin Cartridge Summary

Simple Plex assay is for the detection of human Erythropoietin (Epo) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Erythropoietin/EPO Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human EPO.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Epo assay.

Inter-Assay Precision (Precision between assays) Replicates of each Epo control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Epo.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 9 8
Mean (mIU/mL) 2.65 142 2.75 150
Standard Deviation 0.096 5.62 0.213 9.44
CV% 3.6 4.0 7.8 6.3

Recovery for Simple Plex Human Erythropoietin Cartridge

Recovery for the human Epo assays was evaluated at three different spiked concentrations of Epo protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 108 106-111
EDTA Plasma (n=4) 115 97-125
Heparin Plasma (n=4) 113 97-129
Serum (n=4) 113 111-115

Linearity

Samples containing and/or spiked with high concentrations of human EPO were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Epo Assay Linearity

Scientific Data Images for Simple Plex Human Erythropoietin Cartridge

Simple Plex Human Epo Assay Standard Curve

Simple Plex Human Epo Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Epo Assay. Assay range is 0.36-550 mIU/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.

R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

Additional Erythropoietin/EPO Products

Product Documents for Simple Plex Human Erythropoietin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Erythropoietin Cartridge

For research use only

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