Simple Plex Human FGF-21 Cartridge
R&D Systems | Catalog # SPCKB-PS-000401
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human FGF-21 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 22 |
| Mean (pg/mL) | 34.9 | 1705 | 33.6 | 1654 |
| Standard Deviation | 2.86 | 131 | 2.63 | 60.1 |
| CV% | 8.2 | 7.7 | 7.8 | 3.6 |
Recovery for Simple Plex Human FGF-21 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 111 | 106-119 |
| EDTA Plasma (n=4) | 107 | 99-113 |
| Heparin Plasma (n=4) | 108 | 97-122 |
| Serum (n=4) | 97 | 85-103 |
Linearity
Samples containing and/or spiked with high concentrations of human FGF-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human FGF-21 Cartridge
Human FGF-21 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
Long Name
Alternate Names
Gene Symbol
Additional FGF-21 Products
Product Documents for Simple Plex Human FGF-21 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human FGF-21 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Human FGF-21 Cartridge
View all citations for the Simple Plex platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
Powered by Bioz
Customer Reviews for Simple Plex Human FGF-21 Cartridge
There are currently no reviews for this product. Be the first to review Simple Plex Human FGF-21 Cartridge and earn rewards!
Have you used Simple Plex Human FGF-21 Cartridge?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review