Simple Plex Human FGF-21 Cartridge

R&D Systems | Catalog # SPCKB-PS-000401

Simple Plex Human FGF-21 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

3.74 pg/mL

Assay Range

8.93-5450 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human FGF-21 Cartridge Summary

Simple Plex assay is for the detection of human Fibroblast Growth Factor 21 (FGF-21) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all FGF-21 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human FGF-21.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FGF-21 assay.

Inter-Assay Precision (Precision between assays) Replicates of each FGF-21 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FGF-21.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 22
Mean (pg/mL) 34.9 1705 33.6 1654
Standard Deviation 2.86 131 2.63 60.1
CV% 8.2 7.7 7.8 3.6

Recovery for Simple Plex Human FGF-21 Cartridge

Recovery for the human FGF-21 assays was evaluated at three different spiked concentrations of FGF-21 protein.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 111 106-119
EDTA Plasma (n=4) 107 99-113
Heparin Plasma (n=4) 108 97-122
Serum (n=4) 97 85-103

Linearity

Samples containing and/or spiked with high concentrations of human FGF-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human FGF-21 Assay Linearity

Scientific Data Images for Simple Plex Human FGF-21 Cartridge

Simple Plex Human FGF-21 Assay Standard Curve

Simple Plex Human FGF-21 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FGF-21 Assay. Assay range is 8.93-5450 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-21

FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.

Long Name

Fibroblast Growth Factor 21

Alternate Names

FGF21

Entrez Gene IDs

26291 (Human); 56636 (Mouse)

Gene Symbol

FGF21

Additional FGF-21 Products

Product Documents for Simple Plex Human FGF-21 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human FGF-21 Cartridge

For research use only

Citations for Simple Plex Human FGF-21 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human FGF-21 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human FGF-21 Cartridge and earn rewards!

Have you used Simple Plex Human FGF-21 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card