Simple Plex Human Follistatin Cartridge

R&D Systems | Catalog # SPCKB-PS-000489

Simple Plex Human Follistatin assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (5 µL), Serum (5 µL), EDTA Plasma (5 µL)

Sensitivity

0.6 pg/mL

Assay Range

2.77-26410 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Simple Plex Human Follistatin Cartridge Summary

Simple Plex assay is for the detection of human Follistatin in cell culture supernatant (CCS), serum, and EDTA plasma in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Follistatin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Follistatin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Follistatin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Follistatin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Follistatin.

Cell Culture Supernates, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 35 34
Mean (pg/mL) 144 6411 149 6694
Standard Deviation 8.41 572 17.2 733
CV% 5.8 8.9 11.5 11.0

Recovery for Simple Plex Human Follistatin Cartridge

Recovery was not necessary as human Follistatin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Follistatin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human Follistatin Assay Linearity

Scientific Data Images for Simple Plex Human Follistatin Cartridge

Simple Plex Human Follistatin Assay Standard Curve

Simple Plex Human Follistatin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Follistatin Assay. Assay range is 2.77-26,410 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Follistatin

Follistatin (FST) is a secreted glycoprotein that binds and neutralizes several TGF-beta superfamily proteins including Activin, BMP-6, BMP-7, and GDF-8/Myostatin. It also binds Angiogenin and regulates hematopoietic stem cell adhesion to Fibronectin. Some Follistatin binding partners will also bind Follistatin-like proteins such as FSL-3. Of three follistatin isoforms, the full-length mature follistatin (FST315) is the most abundant and the sole form in plasma, but has lower binding affinity for both activins and heparins than alternative forms. The acidic tail is missing in the splice variant FST288, which shows highest affinity, and partial in the proteolytically produced FST303, which shows intermediate affinity. Genetic deletion of follistatin in mice, or expression of only the FST288 form, is lethal perinatally due to defects of lung, skin and musculoskeletal system. Expression of only the FST315 isoform allows survival, with defects in vascularization and female fertility.

Alternate Names

FS, FST

Entrez Gene IDs

10468 (Human); 14313 (Mouse)

Gene Symbol

FST

Additional Follistatin Products

Product Documents for Simple Plex Human Follistatin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Follistatin Cartridge

For research use only

Citations for Simple Plex Human Follistatin Cartridge

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