Simple Plex Human G-CSF Cartridge

R&D Systems | Catalog # SPCKB-PS-002224

Simple Plex Human G-CSF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD10, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.89 pg/mL

Assay Range

3.13-11936 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human G-CSF Cartridge Summary

Simple Plex assay is for the detection of human Granulocyte Colony Stimulating Factor (G-CSF) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD10.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all G-CSF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human G-CSF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one G-CSF assay.

Inter-Assay Precision (Precision between assays) Replicates of each G-CSF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human G-CSF.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 58 58
Mean (pg/mL) 89.7 3851 84.8 3999
Standard Deviation 3.36 277 8.81 455
CV% 3.7 7.2 10.4 11.4

Recovery for Simple Plex Human G-CSF Cartridge

Recovery for the human G-CSF assays was evaluated at three different spiked concentrations of G-CSF protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 97 93-100
Heparin Plasma (n=4) 95 93-98
Serum (n=4) 101 98-106

Linearity

Samples containing and/or spiked with high concentrations of human G-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human G-CSF Assay Linearity

Scientific Data Images for Simple Plex Human G-CSF Cartridge

Simple Plex Human G-CSF Assay Standard Curve

Simple Plex Human G-CSF Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human G-CSF Assay. Assay range is 3.13-11,936 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Long Name

Granulocyte Colony Stimulating Factor

Alternate Names

C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin

Entrez Gene IDs

1440 (Human); 12985 (Mouse)

Gene Symbol

CSF3

Additional G-CSF Products

Product Documents for Simple Plex Human G-CSF Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human G-CSF Cartridge

For research use only

Citations for Simple Plex Human G-CSF Cartridge

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