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Simple Plex Human Galectin-3 Cartridge

Catalog #: SPCKB-PS-000490 Datasheet / COA / SDS
Simple Plex Human Galectin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000490
SPCKA-PS-000490
SPCKC-PS-000490
Control Products Available
898088: Simple Plex Control for Human Galectin-3
Human Galectin-3 Simple Plex Assay
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Galectin-3 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 nL), Serum (500 nL), EDTA Plasma (500 nL), Heparin Plasma (500 nL)
Sensitivity
1 pg/mL
Assay Range
3.1 - 4,740 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Galectin-3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Galectin-3 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

Recovery

Recovery was not necessary as Galectin-3 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Galectin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Galectin-3 Simple Plex Assay Linearity

Scientific Data

N/A Human Galectin-3 Simple Plex Assay Standard Curve View Larger

Human Galectin-3 Simple Plex Assay Standard Curve

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Product Datasheet
COA
SDS

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.

Entrez Gene IDs:
3958 (Human); 16854 (Mouse)
Alternate Names:
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

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