Simple Plex Human Galectin-3 Cartridge

R&D Systems | Catalog # SPCKB-PS-000490

Simple Plex Human Galectin-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

1 pg/mL

Assay Range

3.11-4740 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human Galectin-3 Cartridge Summary

Simple Plex assay for the detection of human Galectin-3 in cell culture supernates (CCS), serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Galectin-3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Galectin-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 17 19
Mean (pg/mL) 34.2 1700 29.8 1593
Standard Deviation 2.57 129 2.75 170
CV% 7.5 7.6 9.2 10.7

Recovery for Simple Plex Human Galectin-3 Cartridge

Recovery was not necessary as Galectin-3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Galectin-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Galectin-3 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human Galectin-3 Cartridge

Human Galectin-3 Simple Plex Assay Standard Curve

Human Galectin-3 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35, and etaBP, is a secreted lectin that acts in anti-microbial immunity  by pathogen opsonization, macrophage recruitment, and the activation of mast cells and neutrophils. It can also contribute to chronic inflammation and fibrosis. It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis.

Alternate Names

AGE-R3, CBP35, GAL3, Galectin3, L29, LGALS3, Mac-2

Entrez Gene IDs

3958 (Human); 16854 (Mouse)

Gene Symbol

LGALS3

Additional Galectin-3 Products

Product Documents for Simple Plex Human Galectin-3 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human Galectin-3 Cartridge

For research use only

Citations for Simple Plex Human Galectin-3 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human Galectin-3 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human Galectin-3 Cartridge and earn rewards!

Have you used Simple Plex Human Galectin-3 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card