Simple Plex Human HGF Cartridge
R&D Systems | Catalog # SPCKB-PS-000143
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human HGF Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 9 |
| Mean (pg/mL) | 87.1 | 4038 | 89.3 | 4416 |
| Standard Deviation | 5.03 | 339 | 3.24 | 316 |
| CV% | 5.8 | 8.4 | 3.6 | 7.2 |
Recovery for Simple Plex Human HGF Cartridge
Recovery was not necessary as HGF shows natural linearity data from a neat sample to a 1:16 dilution
Linearity
Samples
containing and/or spiked with high concentrations of human HGF were serially
diluted with Sample Diluent to produce samples within the dynamic range of the
assay
Scientific Data Images for Simple Plex Human HGF Cartridge
Human HGF Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: HGF
HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional HGF Products
Product Documents for Simple Plex Human HGF Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human HGF Cartridge
For research use only
Related Research Areas
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Associated Pathways
