Simple Plex Human IL-18 BPa Cartridge

Catalog #: SPCKB-PS-000502 Datasheet / COA / SDS
Simple Plex Human IL-18 BPa assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000502
SPCKA-PS-000502
SPCKC-PS-000502
Control Products Available
Human IL-18 BPa Simple Plex Assay
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Simple Plex Human IL-18 BPa Cartridge Summary

Sample Type & Volume Required Per Well*
Bronchoalveolar Lavage Fluid (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.881 pg/mL
Assay Range
2.8 - 4,336 pg/mL (Bronchoalveolar Lavage Fluid, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-18 BPa.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human IL-18 BPa in serum, plasma (EDTA/Heparin), and bronchoalveolar lavage fluid (BALF).

Recovery

Recovery was not necessary as IL-18 BPa shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human IL-18 BPa were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data

Human IL-18 BPa Ella Assay Standard Curve

Human IL-18 BPa Ella Assay Linearity

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18 BPa

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

Long Name:
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs:
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names:
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa

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