Simple Plex Human IL-22 Cartridge
R&D Systems | Catalog # SPCKB-PS-001529
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-22 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-22 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-22 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-22.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 56 | 56 |
| Mean (pg/mL) | 426 | 21,967 | 449 | 22,902 |
| Standard Deviation | 29.1 | 847 | 42.2 | 1579 |
| CV% | 6.8 | 3.9 | 9.4 | 6.9 |
Recovery for Simple Plex Human IL-22 Cartridge
Recovery for the human IL-22 assays was evaluated at three different spiked concentrations of IL-22 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 96 | 89-99 |
| Heparin Plasma (n=4) | 91 | 87-101 |
| Serum (n=4) | 98 | 94-103 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-22 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-22 Cartridge
Simple Plex Human IL-22 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-22 Assay. Assay range is 16.6-63,300 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
Long Name
Alternate Names
Gene Symbol
Additional IL-22 Products
Product Documents for Simple Plex Human IL-22 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human IL-22 Cartridge
For research use only
Related Research Areas
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