Simple Plex Human IL-7 Cartridge

Catalog #: SPCKB-PS-000506 Datasheet / COA / SDS
Simple Plex Cartridge Kit for quantitation of IL-7. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system
Catalog # Availability Size / Price Qty
SPCKB-PS-000506
SPCKA-PS-000506
SPCKC-PS-000506
Control Products Available
Human IL-7 Simple Plex Assay
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Simple Plex Human IL-7 Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.57 pg/mL
Assay Range
0.6 - 2,170 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-7.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Summary

Simple Plex assay for the detection of human IL-7 in cell culture supernatan (CCS), serum, and plasma (EDTA/Heparin)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 1
n 16 16 16 16
Mean (pg/mL) 11.9 622 12 628
Standard Deviation 0.454 35.5 0.58 28.7
CV% 3.8 5.7 4.8 4.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 95 90-102
Heparin Plasma (n=4) 76 70-84
Serum (n=4) 96 90-101

Linearity

Samples containing and/or spiked with high concentrations of human IL-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human IL-7 Simple Plex Assay Linearity

Scientific Data

Human IL-7 Simple Plex Assay Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Long Name:
Interleukin 7
Entrez Gene IDs:
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names:
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

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