Simple Plex Human KGF/FGF-7 Cartridge
R&D Systems | Catalog # SPCKB-PS-001015
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human KGF/FGF-7 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one KGF/FGF-7 assay.
Inter-Assay Precision (Precision between assays) Replicates of each KGF/FGF-7 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human KGF/FGF-7.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 17 | 17 |
| Mean (pg/mL) | 41.9 | 2042 | 40.8 | 2122 |
| Standard Deviation | 1.79 | 144 | 3.10 | 125 |
| CV% | 4.3 | 7.1 | 7.6 | 5.9 |
Recovery for Simple Plex Human KGF/FGF-7 Cartridge
Recovery for the human KGF/FGF-7 assays was evaluated at three different spiked concentrations of KGF/FGF-7 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 106 | 97-113 |
| EDTA Plasma (n=4) | 99 | 92-104 |
| Heparin Plasma (n=4) | 96 | 92-99 |
| Serum (n=4) | 107 | 100-110 |
Linearity
Samples containing and/or spiked with high concentrations of human KGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human KGF/FGF-7 Cartridge
Simple Plex Human KGF/FGF-7 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human KGF/FGF-7 Assay. Assay range is 1.80-6850 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: KGF/FGF-7
KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.
Long Name
Alternate Names
Gene Symbol
Additional KGF/FGF-7 Products
Product Documents for Simple Plex Human KGF/FGF-7 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human KGF/FGF-7 Cartridge
For research use only
Related Research Areas
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Associated Pathways
