Simple Plex Human LAG-3 Cartridge
R&D Systems | Catalog # SPCKB-PS-001348
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human LAG-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 61 | 60 |
| Mean (pg/mL) | 936 | 43772 | 972 | 45960 |
| Standard Deviation | 47.9 | 3730 | 81.5 | 4953 |
| CV% | 5.1 | 8.5 | 8.4 | 10.8 |
Recovery for Simple Plex Human LAG-3 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 102 | 101-102 |
| EDTA Plasma (n=4) | 105 | 97-110 |
| Heparin Plasma (n=4) | 102 | 92-114 |
| Serum (n=4) | 97 | 95-98 |
Linearity
Samples containing and/or spiked with high concentrations of human LAG-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human LAG-3 Cartridge
Human LAG-3 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: LAG-3
LAG-3 (Lymphocyte activation gene-3; CD223) is a transmembrane protein that binds to MHC class II molecules and negatively regulates T cell receptor signaling. It is expressed on activated T cells, NK cells, and plasmacytoid dendritic cells (pDC). LAG-3 limits the expansion of activated T cells and pDC in response to select stimuli. Proteolytic shedding of LAG-3 enables normal T cell activation by removing the negative regulation. Binding of a homodimerized soluble LAG-3/Ig fusion protein to MHC class II molecules induces maturation of immature DC as well as secretion of pro-inflammatory cytokines by cytotoxic CD8+ T cells and NK cells.
Long Name
Alternate Names
Gene Symbol
Additional LAG-3 Products
Product Documents for Simple Plex Human LAG-3 Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human LAG-3 Cartridge
For research use only
Related Research Areas
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