Simple Plex Human LIGHT Cartridge

R&D Systems | Catalog # SPCKB-PS-000142

Simple Plex Human LIGHT/TNFSF14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.314 pg/mL

Assay Range

1.07-4070 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human LIGHT Cartridge Summary

Simple Plex assay is for the detection of human LIGHT in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all LIGHT/TNFSF14 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human LIGHT.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one LIGHT assay.

Inter-Assay Precision (Precision between assays) Replicates of each LIGHT control were tested in multiple assays performed by at least three technicians using two lots of reagents for human LIGHT.

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 23 22
Mean (pg/mL) 22.2 1109 20.1 1152
Standard Deviation 0.652 59.8 1.43 64.7
CV% 2.9 5.4 7.1 5.6

Recovery for Simple Plex Human LIGHT Cartridge

Recovery for the human LIGHT assays was evaluated at three different spiked concentrations of LIGHT protein.

Sample Type Average % Recovery Range %
Cerebrospinal Fluid (n=6) 106 101-115
EDTA Plasma (n=4) 97 84-103
Heparin Plasma (n=4) 105 100-111
Serum (n=4) 100 86-112

Linearity

Samples containing and/or spiked with high concentrations of human LIGHT were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human LIGHT Assay Linearity

Scientific Data Images for Simple Plex Human LIGHT Cartridge

Simple Plex Human LIGHT Assay Standard Curve

Simple Plex Human LIGHT Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human LIGHT Assay. Assay range is 1.07-4070 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIGHT/TNFSF14

LIGHT, also known as TNFSF14, is a homotrimeric glycoprotein in the TNF superfamily. It is expressed on the surface of activated lymphocytes, NK cells, immature dendritic cells, monocytes, and granulocytes. A soluble form can be shed by proteolysis. LIGHT binds and activates HVEM/TNFRSF14 to costimulate Th1 immune responses, enhance CD8+ T cell‑mediated tumor immunity, and regulate allogeneic T cell activation and allograft rejection. In addition, it binds and activates Lymphotoxin beta R/TNFRSF3 to induce apoptosis, cytokine production, mesenteric lymph node organogenesis, and maintenance of lymph node structure and function. It can also bind the soluble human decoy receptor DcR3/TNFRSF6B.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human); 50930 (Mouse)

Gene Symbol

TNFSF14

Additional LIGHT/TNFSF14 Products

Product Documents for Simple Plex Human LIGHT Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human LIGHT Cartridge

For research use only

Citations for Simple Plex Human LIGHT Cartridge

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