Simple Plex Human Nectin-2 Cartridge

R&D Systems | Catalog # SPCKB-PS-006536

Simple Plex Human Nectin-2/CD112 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.51 pg/mL

Assay Range

6.55-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Nectin-2 Cartridge Summary

Simple Plex assay is for the detection of human Nectin-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Nectin-2/CD112 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Specificity

Natural and recombinant human Nectin-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Nectin-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each Nectin-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Nectin-2.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 38 38
Mean (pg/mL) 29.7 1618 29.2 1417
Standard Deviation 2.47 131.9 2.79 105
CV% 8.3 8.2 9.6 7.4

Recovery for Simple Plex Human Nectin-2 Cartridge

Recovery was not necessary as human Nectin-2 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Nectin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Nectin-2 Assay Linearity

Scientific Data Images for Simple Plex Human Nectin-2 Cartridge

Simple Plex Human Nectin-2 Assay Standard Curve

Simple Plex Human Nectin-2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Nectin-2 Assay. Assay range is 6.55-4000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Nectin-2/CD112

Nectins are type I transmembrane glycoproteins that are calcium-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs). Homology has led to their designation as poliovirus receptor-related (PRR) proteins, but nectins do not bind poliovirus. The extracellular domains of the nectin family form cis-homodimers (same cell), followed by homophilic or heterophilic trans-dimers (across cells) that organize intercellular junctions. Nectin-1, also known as CD111, PRR1 and herpes virus entry mediator C (HVEC) has a secreted splice variant. Nectin-1, Nectin-2 (CD112, PRR2, HVEB) and Nectin-3 (PRR3) are concentrated in adherens junctions and exist on neurons, endothelial cells, epithelial cells and fibroblasts. Nectin-4 is mainly expressed in the placenta; a soluble form is generated via the action of TACE/ADAM17.

Long Name

Poliovirus Receptor Related 2

Alternate Names

CD112, HVEB, MPH, Nectin2, PRR2, PVRL2

Entrez Gene IDs

5819 (Human); 19294 (Mouse); 102137456 (Cynomolgus Monkey)

Gene Symbol

NECTIN2

Additional Nectin-2/CD112 Products

Product Documents for Simple Plex Human Nectin-2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Nectin-2 Cartridge

For research use only

Citations for Simple Plex Human Nectin-2 Cartridge

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