Simple Plex Human Neutrophil Elastase/ELA2 Cartridge

R&D Systems | Catalog # SPCKB-PS-011934

Simple Plex Human Neutrophil Elastase/ELA2 assay kit for use on Ella instrument. Contains a cartridge, Sample Diluent SD06, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

3.97 pg/mL

Assay Range

16.38-10000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Neutrophil Elastase/ELA2 Cartridge Summary

Simple Plex assay for the detection of human Human Neutrophil Elastase (ELA2) in cell culture supernates (CCS), serum, and plasma (EDTA/Heparin). 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Neutrophil Elastase/ELA2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human ELA2. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 78 53
Mean (pg/mL) 63.9 4161 68.5 3515
Standard Deviation 2.39 203 8.10 325
CV% 3.7 4.9 11.9 9.3

Recovery for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 110 109-110

Linearity

Samples containing and/or spiked with high concentrations of human ELA2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

Scientific Data Images for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge

Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

Simple Plex Human Neutophil Elastase/ELA2 Ella Assay

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Entrez Gene IDs

1991 (Human); 50701 (Mouse)

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Neutrophil Elastase/ELA2 Cartridge

For research use only

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