Simple Plex Human Serpin A1 Cartridge
R&D Systems | Catalog # SPCKB-PS-001047
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Serpin A1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 27 | 31 |
| Mean (pg/mL) | 463 | 18669 | 475 | 19781 |
| Standard Deviation | 18.8 | 1679 | 34.7 | 2004 |
| CV% | 4.1 | 9.0 | 7.3 | 10.1 |
Recovery for Simple Plex Human Serpin A1 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=2) | 100 | 93-108 |
Linearity
Samples containing and/or spiked with high concentrations of human Serpin A1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Serpin A1 Cartridge
Human Serpin A1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Serpin A1/alpha 1-Antitrypsin
The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional Serpin A1/alpha 1-Antitrypsin Products
Product Documents for Simple Plex Human Serpin A1 Cartridge
Certificate of Analysis
To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.
Product Specific Notices for Simple Plex Human Serpin A1 Cartridge
For research use only
Related Research Areas
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