Simple Plex Human TACI/TNFRSF13B Cartridge

Catalog #: SPCKB-PS-000381 Datasheet / COA / SDS

Simple Plex Human TACI/TNFRSF13B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer

Catalog #	Availability	Size / Price	Qty
SPCKB-PS-000381
SPCKA-PS-000381
SPCKC-PS-000381

Control Products Available

898414: Simple Plex Control for Human TACI/TNFRSF13B

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human TACI/TNFRSF13B Cartridge Summary

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)

Sensitivity

0.534 pg/mL

Assay Range

2.5 - 24,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Specificity

Natural and recombinant human TACI.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human TACI in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF)

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	15	15
Mean (µL)	32.7	2845	33.4	3209
Standard Deviation	0.912	90.6	2.56	255
CV%	2.8	3.2	7.7	7.9

Recovery

Recovery was not necessary as TACI shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human TACI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data

N/A

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Human TACI/TNFRSF13B Ella Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily (TNFRSF). Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA both bind APRIL and BLyS/BAFF, members of the TNF superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Human and mouse TACI share 54% amino acid sequence identity.

Long Name:

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs:

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names:

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B