Simple Plex Human TACI/TNFRSF13B Cartridge

R&D Systems | Catalog # SPCKB-PS-000381

Simple Plex Human TACI/TNFRSF13B assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Cerebrospinal Fluid (25 µL)

Sensitivity

0.534 pg/mL

Assay Range

2.52-24000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)

Simple Plex Human TACI/TNFRSF13B Cartridge Summary

Simple Plex assay is for the detection of human Transmembrane Activator and CAML-Interactor (TACI) in serum, plasma (EDTA/Heparin), and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TACI/TNFRSF13B Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TACI.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TACI assay.

Inter-Assay Precision (Precision between assays) Replicates of each TACI control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TACI.

Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 15 15
Mean (µL) 32.7 2845 33.4 3209
Standard Deviation 0.912 90.6 2.56 255
CV% 2.8 3.2 7.7 7.9

Recovery for Simple Plex Human TACI/TNFRSF13B Cartridge

Recovery was not necessary as human TACI shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human TACI were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human TACI Assay Linearity

Scientific Data Images for Simple Plex Human TACI/TNFRSF13B Cartridge

Simple Plex Human TACI Assay Standard Curve

Simple Plex Human TACI Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TACI Assay. Assay range is 2.52-24,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily (TNFRSF). Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA both bind APRIL and BLyS/BAFF, members of the TNF superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Human and mouse TACI share 54% amino acid sequence identity.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Alternate Names

CD267, CVID, TNFRSF13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Gene Symbol

TNFRSF13B

Additional TACI/TNFRSF13B Products

Product Documents for Simple Plex Human TACI/TNFRSF13B Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human TACI/TNFRSF13B Cartridge

For research use only

Citations for Simple Plex Human TACI/TNFRSF13B Cartridge

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