Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

R&D Systems | Catalog # SPCKB-PS-002900

Simple Plex TIM-1/KIM-1/HAVCR assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL)

Sensitivity

0.55 pg/mL

Assay Range

3.41-5200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge Summary

Simple Plex assay is for the detection of human T cell Immunoglobulin-Mucin (TIM-1) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TIM-1/KIM-1/HAVCR Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TIM-1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIM-1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TIM-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TIM-1.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 56 59
Mean (pg/mL) 36.5 1941 37.2 1853
Standard Deviation 3.35 93.5 4.49 185
CV% 9.2 4.8 12.1 10.0

Recovery for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Recovery for the human TIM-1 assays was evaluated at three different spiked concentrations of TIM-1 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 96 91-102
Heparin Plasma (n=4) 93 89-98
Serum (n=4) 104 97-108

Linearity

Samples containing and/or spiked with high concentrations of human TIM-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Human TIM-1 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

Simple Plex Human TIM-1/KIM-1/HAVCR Assay Standard Curve

Simple Plex Human TIM-1/KIM-1/HAVCR Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TIM-1/KIM-1/HAVCR Assay. Assay range is 3.41-5200 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.

Long Name

T Cell Immunoglobulin Mucin-1

Alternate Names

CD365, HAVCR1, KIM-1, TIM1

Entrez Gene IDs

26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)

Gene Symbol

HAVCR1

Additional TIM-1/KIM-1/HAVCR Products

Product Documents for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

For research use only

Citations for Simple Plex Human TIM-1/KIM-1/HAVCR Cartridge

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