Simple Plex Human TIM-3 Cartridge

R&D Systems | Catalog # SPCKB-PS-001049

Simple Plex Human TIM-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.33 pg/mL

Assay Range

2.04-7780 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human TIM-3 Cartridge Summary

Simple Plex assay is for the detection of human T cell Immunoglobulin and Mucin domain 3 (TIM-3) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all TIM-3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human TIM-3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TIM-3 assay.

Inter-Assay Precision (Precision between assays) Replicates of each TIM-3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TIM-3.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 22
Mean (pg/mL) 34.0 1645 40.2 1964
Standard Deviation 2.76 125 4.40 196
CV% 8.0 8.0 11.0 10.0

Recovery for Simple Plex Human TIM-3 Cartridge

Recovery was not necessary as human TIM-3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human TIM-3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Human TIM-3 Assay Linearity

Scientific Data Images for Simple Plex Human TIM-3 Cartridge

Simple Plex Human TIM-3 Assay Standard Curve

Simple Plex Human TIM-3 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TIM-3 Assay. Assay range is 2.04-7780 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TIM-3

TIM-3 (T cell immunoglobulin and mucin domain-3), also known as HAVCR2 is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. It is upregulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). TIM-3 ligation by Galectin-9 attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. Dendritic cell-expressed TIM-3 dampens inflammation by enabling the phagocytosis of apoptotic cells and the cross-presentation of apoptotic cell antigens. TIM-3 also binds the alarmin HMGB1, thereby preventing the activation of TLRs in response to released tumor cell DNA.

Long Name

T Cell Immunoglobulin Mucin-3

Alternate Names

CD366, HAVcr-2, HAVCR2, KIM-3, SPTCL, TIM3, TIMD3

Entrez Gene IDs

84868 (Human); 171285 (Mouse); 363578 (Rat); 479318 (Canine); 102141722 (Cynomolgus Monkey)

Gene Symbol

HAVCR2

Additional TIM-3 Products

Product Documents for Simple Plex Human TIM-3 Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human TIM-3 Cartridge

For research use only

Citations for Simple Plex Human TIM-3 Cartridge

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