Simple Plex Human VEGF-D Cartridge
R&D Systems | Catalog # SPCKB-PS-009111
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human VEGF-D Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 43 | 43 |
| Mean (pg/mL) | 93.3 | 4309 | 88.5 | 4192 |
| Standard Deviation | 3.93 | 164 | 8.12 | 399 |
| CV% | 4.2 | 3.8 | 9.2 | 9.5 |
Recovery for Simple Plex Human VEGF-D Cartridge
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 95 | 92-99 |
| EDTA Plasma (n=4) | 90 | 81-101 |
| Heparin Plasma (n=4) | 94 | 90-101 |
| Serum (n=4) | 88 | 77-96 |
Linearity
Samples containing and/or spiked with high concentrations of human VEGF-D were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human VEGF-D Cartridge
Human VEGF-D Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: VEGF-D
Vascular endothelial growth factor D (VEGF-D) is a homodimeric ligand of VEGF R3/Flt-4 and is synthesized with N- and C-terminal propeptides. Fully processed VEGF-D containing only the VEGF homology domain can additionally bind and activate VEGF R2/KDR/Flk-1. VEGF-D is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium. While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, while VEGF-D is most active in neonatal lymphatic maturation and bone growth.
Long Name
Alternate Names
Gene Symbol
Additional VEGF-D Products
Product Documents for Simple Plex Human VEGF-D Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human VEGF-D Cartridge
For research use only
Citations for Simple Plex Human VEGF-D Cartridge
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