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Simple Plex Human VEGFR1/Flt-1 Cartridge

Name: Simple Plex Human VEGFR1/Flt-1 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000540
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000540

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Product Documents

Simple Plex Human VEGFR1/Flt-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.6 pg/mL

Assay Range

3.05-4650 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human VEGFR1/Flt-1 Cartridge Summary

Simple Plex assay is for the detection of human Vascular Endothelial Growth Factor Receptor 1 (VEGFR1) in serum and plasma (EDTA/Heparin) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human VEGF R1.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one VEGFR1 assay.

Inter-Assay Precision (Precision between assays) Replicates of each VEGFR1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human VEGFR1.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	19	19
Mean (pg/mL)	27.1	1312	28.3	1336
Standard Deviation	1.99	123	1.99	119
CV%	7.3	9.4	7.0	8.9

Recovery for Simple Plex Human VEGFR1/Flt-1 Cartridge

Recovery for the human VEGFR1 assays was evaluated at three different spiked concentrations of VEGFR1 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=3)	104	99-111
Heparin Plasma (n=3)	81	73-88
Serum (n=3)	97	79-111

Linearity

Samples containing and/or spiked with high concentra-tions of human VEGF R1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human VEGFR1/Flt-1 Cartridge

Simple Plex Human VEGFR1 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human VEGFR1 Assay. Assay range is 3.05-4650 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR1/Flt-1

VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.