Simple Plex Mouse Angiopoietin-like 3 Cartridge

R&D Systems | Catalog # SPCKB-MP-007716

Simple Plex Mouse Angiopoietin-like 3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

5.66 pg/mL

Assay Range

15.7-24000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse Angiopoietin-like 3 Cartridge Summary

Simple Plex assay is for the detection of mouse Angiopoietin-like 3 (ANGPTL3) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Angiopoietin-like Protein 3/ANGPTL3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse Angiopoietin-like 3.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Replicates of each Angiopoietin-like 3 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse Angiopoietin-like 3.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 60 60
Mean (pg/mL) 146 7688 136 7884
Standard Deviation 6.51 505 16.0 932
CV% 4.5 6.6 11.7 11.8

Recovery for Simple Plex Mouse Angiopoietin-like 3 Cartridge

Recovery was not necessary as mouse Angiopoietin-like 3 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of mouse ANGPTL3 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Mouse Angiopoietin-like 3 Assay Linearity

Scientific Data Images for Simple Plex Mouse Angiopoietin-like 3 Cartridge

Simple Plex Mouse Angiopoietin-like 3 Assay Standard Curve

Simple Plex Mouse Angiopoietin-like 3 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse Angiopoietin-like 3 Assay. Assay range is 15.7-24,000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 3/ANGPTL3

Angiopoietin-like Protein 3 (ANGPTL3) is a secreted glycoprotein that is primarily expressed in the liver. Full length ANGPTL3 circulates in the plasma as do the proteolytically separated N- and C-terminal fragments containing the coiled coil domain and fibrinogen-like domains, respectively. ANGPTL3 directly inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes responsible for hydrolyzing circulating triglycerides and HDL phospholipids. It promotes an increase in circulating triglyceride levels without altering VLDL or HDL secretion or uptake. ANGPTL3 is upregulated by LXR agonists and downregulated by Insulin, Leptin, and agonists of TR beta or PPAR beta. Its fibrinogen-like domain interacts with Integrin alphaV beta3 to induce endothelial cell adhesion, migration, and neovascularization. ANGPTL3 also promotes the expansion of hematopoietic stem cells.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Entrez Gene IDs

27329 (Human); 30924 (Mouse); 102136264 (Cynomolgus Monkey)

Gene Symbol

ANGPTL3

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Simple Plex Mouse Angiopoietin-like 3 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse Angiopoietin-like 3 Cartridge

For research use only

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