Simple Plex Mouse/Rat CCL5/RANTES Cartridge
R&D Systems | Catalog # SPCKB-MP-007962
Customize This Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat CCL5/RANTES Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 108 | 108 |
| Mean (pg/mL) | 26.9 | 1402 | 13.9 | 696 |
| Standard Deviation | 0.621 | 40.9 | 1.17 | 50.5 |
| CV% | 2.3 | 2.9 | 8.4 | 7.3 |
Recovery for Simple Plex Mouse/Rat CCL5/RANTES Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 105 | 101-109 |
Linearity
Samples containing and/or spiked with high concentrations of mouse/rat RANTES were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat CCL5/RANTES Cartridge
Mouse/Rat CCL5/RANTES Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: CCL5/RANTES
CCL5/RANTES is a secreted beta-chemokine that plays a primary role in the inflammatory immune response through chemoattraction and activation of leukocytes that express CCR1, 3, 4, or 5. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1.
Alternate Names
Gene Symbol
Additional CCL5/RANTES Products
Product Documents for Simple Plex Mouse/Rat CCL5/RANTES Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Mouse/Rat CCL5/RANTES Cartridge
For research use only
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Associated Pathways
