Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

R&D Systems | Catalog # SPCKB-MP-001485

Simple Plex Mouse CXCL10/IP-10/CRG-2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (25 µL), Mouse EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL)

Sensitivity

1.37 pg/mL

Assay Range

3.93-6000 pg/mL (Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge Summary

Simple Plex assay is for the detection of mouse Interferon gamma Inducible Protein 10 (CXCL10/IP-10) in serum and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CXCL10/IP-10/CRG-2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse CXCL10/IP-10.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CXCL10/IP-10 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CXCL10/IP-10 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse CXCL10/IP-10.

Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 14 15
Mean (pg/mL) 35.1 1918 36.1 1825
Standard Deviation 1.27 124 2.78 155
CV% 3.6 6.5 7.7 8.5

Recovery for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

Recovery for the mouse CXCL10/IP-10 assays was evaluated at three different spiked concentrations of CXCL10/IP-10 protein.

Sample Type Average % Recovery Range %
Mouse EDTA Plasma (n=4) 113 109-119
Mouse Heparin Plasma (n=4) 106 95-114
Mouse Serum (n=4) 111 103-117

Linearity

Samples containing and/or spiked with high concentrations of mouse CXCL10/IP-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Mouse CXCL10/IP-10 Assay Linearity

Scientific Data Images for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

Simple Plex Mouse CXCL10/IP-10 Assay Standard Curve

Simple Plex Mouse CXCL10/IP-10 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse CXCL10/IP-10 Assay. Assay range is 3.93-6000 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.

Alternate Names

CRG-2, CRG2, IP-10

Entrez Gene IDs

3627 (Human); 15945 (Mouse)

Gene Symbol

CXCL10

Additional CXCL10/IP-10/CRG-2 Products

Product Documents for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

For research use only

Citations for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge and earn rewards!

Have you used Simple Plex Mouse CXCL10/IP-10/CRG-2 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card