Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Catalog #: SPCKB-MP-000583 Datasheet / COA / SDS
Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
Control Products Available
Simple Plex Cartridge Kit for 16 samples, 1 analyte for use with Mouse Plasma / Serum.
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Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary

Sample Type & Volume Required Per Well*
Mouse Cell Culture Supernates (500 nL), Mouse Serum (25 uL), Mouse EDTA Plasma (25 uL), Mouse Heparin Plasma (25 uL)
0.92 pg/mL
Assay Range
0.9 - 1,412 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)
Natural and recombinant mouse MIP-2.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse MIP-2 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 10.9 475 10.3 463
Standard Deviation 0.994 40.8 1.2 39.3
CV% 9.1 8.6 11.6 8.5


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Mouse EDTA Plasma (n=4) 106 100-115
Mouse Heparin Plasma (n=4) 107 100-116
Mouse Serum (n=4) 104 94-113


Samples containing and/or spiked with high concentrations of mouse MIP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Mouse CXCL2/MIP-2 Simple Plex Assay Linearity

Scientific Data

Mouse CXCL2/MIP-2 Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Entrez Gene IDs:
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2


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