Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

R&D Systems | Catalog # SPCKB-MP-000583

Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Cell Culture Supernates (10 µL), Mouse Serum (25 µL), Mouse EDTA Plasma (25 µL), Mouse Heparin Plasma (25 µL)

Sensitivity

0.06 pg/mL

Assay Range

0.92-1412 pg/mL (Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma)

Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary

Simple Plex assay is for the detection of mouse Macrophage Inflammatory Protein 2 (CXCL2/MIP-2) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CXCL2/GRO beta/MIP-2/CINC-3 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse CXCL2/MIP-2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CXCL2/MIP-2 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CXCL2/MIP-2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse CXCL2/MIP-2.

Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 91 92
Mean (pg/mL) 10.9 475 10.3 463
Standard Deviation 0.994 40.8 1.20 39.3
CV% 9.1 8.6 11.6 8.5

Recovery for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Recovery for the mouse CXCL2/MIP-2 assays was evaluated at three different spiked concentrations of CXCL2/MIP-2 protein.

Sample Type Average % Recovery Range %
Mouse EDTA Plasma (n=4) 106 100-115
Mouse Heparin Plasma (n=4) 107 100-116
Mouse Serum (n=4) 104 94-113

Linearity

Samples containing and/or spiked with high concentrations of mouse CXCL2/MIP-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Mouse CXCL2/MIP-2 Assay Linearity

Scientific Data Images for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

Simple Plex Mouse CXCL2/MIP-2 Assay Standard Curve

Simple Plex Mouse CXCL2/MIP-2 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse CXCL2/MIP-2 Assay. Assay range is 0.92-1412 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Alternate Names

CINC-3, CINC3, GRO beta, MGSA-beta, MIP-2

Entrez Gene IDs

2920 (Human); 20310 (Mouse); 114105 (Rat)

Gene Symbol

CXCL2

Additional CXCL2/GRO beta/MIP-2/CINC-3 Products

Product Documents for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge

For research use only

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