Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge
Simple Plex Mouse CXCL2/GRO beta/MIP-2/CINC-3 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
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Mouse Cell Culture Supernates, Mouse Serum, Mouse EDTA Plasma, Mouse Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
Recovery at three different spiked concentrations within the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Mouse EDTA Plasma (n=4)||106||100-115|
|Mouse Heparin Plasma (n=4)||107||100-116|
|Mouse Serum (n=4)||104||94-113|
Mouse CXCL2/MIP-2 Simple Plex Assay Standard Curve
Preparation and Storage
Background: CXCL2/GRO beta/MIP-2/CINC-3
CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.
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