Simple Plex Mouse Erythropoietin/EPO Cartridge

R&D Systems | Catalog # SPCKB-MP-009096

Simple Plex Mouse EPO assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

1.03 pg/mL

Assay Range

7.86-12000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse Erythropoietin/EPO Cartridge Summary

Simple Plex assay for the detection of mouse Erythropoietin (Epo) in cell culture supernatant (CCS), serum and plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Erythropoietin/EPO Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse Epo. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 75 75
Mean (pg/mL) 77.0 3917 91.6 4348
Standard Deviation 2.13 96.2 10.8 363
CV% 2.8 2.5 11.8 8.3

Recovery for Simple Plex Mouse Erythropoietin/EPO Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 103 103-103
EDTA Plasma (n=4) 94 89-100
Heparin Plasma (n=4) 83 77-90
Serum (n=4) 100 92-106

Linearity

Samples containing and/or spiked with high concentrations of mouse Epo were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse Erythropoietin/EPO Cartridge

Mouse Erythropoietin/EPO Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Erythropoietin/EPO

Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.

R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

Additional Erythropoietin/EPO Products

Product Documents for Simple Plex Mouse Erythropoietin/EPO Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse Erythropoietin/EPO Cartridge

For research use only

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