Simple Plex Mouse GDF-8/Myostatin Cartridge

R&D Systems | Catalog # SPCKB-MP-012979

Simple Plex Mouse GDF-8 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD63, and Wash Buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

0.41 pg/mL

Assay Range

1.9-18091 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse GDF-8/Myostatin Cartridge Summary

Simple Plex assay for the detection of mouse Growth Differentiation Factor 8 (GDF-8) in serum and plasma (EDTA/Heparin) in Sample Diluent SD63. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GDF-8/Myostatin Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
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Product Specifications

Species

Mouse

Specificity

Natural and recombinant GDF-8. 

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Activation required, see product datasheet.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 68 65
Mean (pg/mL) 224 9507 140 6355
Standard Deviation 6.30 460 14.9 574
CV% 2.8 4.8 10.7 9.0

Recovery for Simple Plex Mouse GDF-8/Myostatin Cartridge

Recovery was not necessary as mouse GDF-8 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of GDF-8 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Mouse GDF-8 Ella Assay

Scientific Data Images for Simple Plex Mouse GDF-8/Myostatin Cartridge

Simple Plex Mouse GDF-8 Ella Assay

Simple Plex Mouse GDF-8 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GDF-8/Myostatin

GDF-8/Myostatin is a secreted protein that is expressed in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass. It is upregulated in mechanically-stressed cardiomyocytes and induces the skeletal muscle wasting which is common in heart failure. The GDF-8 propeptide is cleaved from GDF-8 but remains associated and functions as an inhibitor of mature GDF-8. GDF-8 activity can also be inhibited through its association with Follistatin, FLRG, Decorin, or GASP-1. Active GDF-8 signals through a receptor complex containing Activin RIIB and either Activin RIB/ALK-4 or TGF-beta RI/ALK-5.

Long Name

Growth Differentiation Factor 8

Alternate Names

GDF8, MSLHP, MSTN, Myostatin

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Gene Symbol

MSTN

Additional GDF-8/Myostatin Products

Product Documents for Simple Plex Mouse GDF-8/Myostatin Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse GDF-8/Myostatin Cartridge

For research use only

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