Simple Plex Mouse IL-17 Cartridge

Name: Simple Plex Mouse IL-17 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-003352
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-003352

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Product Documents

Simple Plex Mouse IL-17/IL-17A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.23 pg/mL

Assay Range

1.5-5715 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse IL-17 Cartridge Summary

Simple Plex assay is for the detection of mouse Interleukin 17 (IL-17) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IL-17.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-17 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-17 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-17.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	56	56
Mean (pg/mL)	30.3	1561	35.8	1761
Standard Deviation	1.34	41.0	2.76	144
CV%	4.4	2.6	7.7	8.2

Recovery for Simple Plex Mouse IL-17 Cartridge

Recovery for the mouse IL-17 assays was evaluated at three different spiked concentrations of IL-17 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	98	90-105
Heparin Plasma (n=4)	109	99-116
Serum (n=4)	97	88-105

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-17 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse IL-17 Cartridge

Simple Plex Mouse IL-17 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-17 Assay. Assay range is 1.50-5715 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.