Simple Plex Mouse IL-7 Cartridge

Name: Simple Plex Mouse IL-7 Cartridge
Brand: R&D Systems
SKU: SPCKB-MP-004018
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-MP-004018

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Product Documents

Simple Plex Mouse IL-7 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

2.1 pg/mL

Assay Range

20.9-31848 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Mouse IL-7 Cartridge Summary

Simple Plex assay is for the detection of mouse Interleukin 7 (IL-7) in serum and plasma (EDTA/Heparin) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse IL-7.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-7 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-7 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-7.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	91	88
Mean (pg/mL)	289	14906	202	9468
Standard Deviation	13.1	567	19.8	953
CV%	4.5	3.8	9.8	10.1

Recovery for Simple Plex Mouse IL-7 Cartridge

Recovery for the mouse IL-7 assays was evaluated at three different spiked concentrations of IL-7 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	110	98-120
Heparin Plasma (n=4)	117	115-120
Serum (n=4)	113	109-116

Linearity

Samples containing and/or spiked with high concentrations of mouse IL-7 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse IL-7 Cartridge

Simple Plex Mouse IL-7 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-7 Assay. Assay range is 20.9-31,848 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naÃ¯ve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.