Simple Plex Mouse Lipocalin-2 Cartridge

R&D Systems | Catalog # SPCKB-MP-007400

Simple Plex Mouse Lipocalin-2/NGAL assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL), Bronchoalveolar Lavage Fluid (10 µL)

Sensitivity

0.9 pg/mL

Assay Range

4.24-2584 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Mouse Lipocalin-2 Cartridge Summary

Simple Plex assay is for the detection of mouse Lipocalin-2/NGAL in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), urine, and bronchoalveolar lavage fluid (BALF) in Sample Diluent SD09.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Lipocalin-2/NGAL Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse

Specificity

Natural and recombinant mouse Lipocalin-2/NGAL.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Lipocalin-2/NGAL assay.

Inter-Assay Precision (Precision between assays) Replicates of each Lipocalin-2/NGAL control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse Lipocalin-2/NGAL.

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 20
Mean (pg/mL) 18.9 928 17.7 918
Standard Deviation 1.36 42.9 1.86 91.7
CV% 7.2 4.6 10.5 10.0

Recovery for Simple Plex Mouse Lipocalin-2 Cartridge

Recovery was not necessary as mouse Lipocalin-2/NGAL shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of Lipocalin-2/NGAL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex Mouse Lipocalin-2/NGAL Assay Linearity

Scientific Data Images for Simple Plex Mouse Lipocalin-2 Cartridge

Simple Plex Mouse Lipocalin-2/NGAL Assay Standard Curve

Simple Plex Mouse Lipocalin-2/NGAL Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse Lipocalin-2/NGAL Assay. Assay range is 4.24-2584 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

Lipocalin-2/NGAL (Neutrophil Gelatinase-associated Lipocalin) is a secreted carrier protein that acts in the innate immune response, differentiation, tumorigenesis, and cell survival. It is induced in macrophages, glial cells, and epithelial cells during inflammation, anemia, and hypoxia. Lipocalin-2 binds catecholate siderophores which are secreted by bacteria and are required for bacterial iron uptake. Lipocalin-2 functions as a bacteriostatic agent in the innate immune response by limiting the bacterial iron supply. Iron uptake by Lipocalin-2 into mammalian cells is important for the regulation of iron-sensitive gene transcription. In the kidney, Lipocalin-2-mediated iron trafficking is required for protection from renal injury.

Long Name

Neutrophil Gelatinase-associated Lipocalin

Alternate Names

24p3, LCN2, Lipocalin2, MSFI, NGAL, Oncogene 24p3, p25, Siderocalin, Uterocalin

Entrez Gene IDs

3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)

Gene Symbol

LCN2

Additional Lipocalin-2/NGAL Products

Product Documents for Simple Plex Mouse Lipocalin-2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Mouse Lipocalin-2 Cartridge

For research use only

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