Simple Plex Mouse NF-L Cartridge

Catalog #: SPCKB-MP-003168 Datasheet / COA / SDS
Simple Plex Mouse NFL assay kit contains cartridge, sample diluent SD13, and wash buffer. Part # starting with ST01 ships in 2-3 days. Sample type indicated with MP (Plasma/Serum)
Catalog # Availability Size / Price Qty
SPCKB-MP-003168
SPCKA-MP-003168
SPCKC-MP-003168
ST01B-MP-003168
Control Products Available
Mouse NF-L Simple Plex Assay
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Simple Plex Mouse NF-L Cartridge Summary

Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity
1.09 pg/mL
Assay Range
2.7 - 10,290 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Specificity
Natural and recombinant NF-L.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse NF-L in serum, plasma (EDTA/Heparin) and cerebrospinal fluid.Simple Plex assays on Ella offer precision multiplexing in a simple format. Preloaded standard curves and hand-free automation reduce human error and variability. Every assay on the Ella menu is highly sensitive and fully validated.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 48 48
Mean (pg/mL) 76.6 3309 80.5 3476
Standard Deviation 3.64 154 8.35 267
CV% 4.8 4.7 10.4 7.7

Linearity

Samples containing and/or spiked with high concentrations of NF-L were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Mouse NF-L Simple Plex Assay Linearity

Scientific Data

Mouse NF-L Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NF-L

Neurofilaments are intermediate filaments that are major components of the neuronal cytoskeleton. Neurofilaments are composed of three subunits: the heavy subunit (NF-H), medium subunit (NF-M), and light subunit (NF-L). Neurofilaments are trimers that always contain the 68 kDa NF-L and variably contain 125 kDa NF-M and 200 kDa NF-H. The human neurofilament heavy chain is a phosphorylated cytoskeletal intermediate filament protein that is expressed in neurons. The neurofilament medium subunit Human NF-M is a phosphorylated glycoprotein that belongs to the type IV intermediate filament family. NF-L is a 68 kDa light chain cytoskeletal intermediate filament protein that associates with the 125 kDa NF-M and the 200 kDa NF-H to form neurofilaments.

All three neurofilament subunits share a common structure, with globular head, alpha-helical rod, and carboxy terminal side-arm domains. The subunits assemble to form 10 nm neurofilaments. Accumulation of neurofilaments is seen in several human diseases including amyotrophic lateral sclerosis (ALS), Parkinsons disease, Alzheimers disease, and diabetic neuropathy.

Long Name:
Neurofilament Protein, Light Chain
Entrez Gene IDs:
4747 (Human)
Alternate Names:
68 kDa neurofilament protein; CMT2E; NEFL; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L protein; neurofilament, light polypeptide; neurofilament-light; NF68; NF68FLJ53642; NFL; NF-L; NFLlight polypeptide 68kDa

FAQs

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