Simple Plex Mouse/Rat FGF-21 Cartridge
R&D Systems | Catalog # SPCKB-MP-006323
Customize My Cartridge on Bio-Techne
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat FGF-21 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Mouse Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 2 | 1 |
| n | 16 | 29 | 16 | 29 |
| Mean (pg/mL) | 27.3 | 1345 | 1439 | 26.2 |
| Standard Deviation | 2.22 | 104 | 132 | 2.39 |
| CV% | 8.1 | 7.7 | 9.2 | 9.1 |
Recovery for Simple Plex Mouse/Rat FGF-21 Cartridge
Recovery was not necessary as mouse/rat FGF-21 shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of mouse/rat FGF-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat FGF-21 Cartridge
Mouse/Rat FGF-21 Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: FGF-21
FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
Long Name
Alternate Names
Gene Symbol
Additional FGF-21 Products
Product Documents for Simple Plex Mouse/Rat FGF-21 Cartridge
Product Specific Notices for Simple Plex Mouse/Rat FGF-21 Cartridge
For research use only
Related Research Areas
Citations for Simple Plex Mouse/Rat FGF-21 Cartridge
View all citations for the Simple Plex platform on Bio-Techne.com.
Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.
Powered by Bioz
Customer Reviews for Simple Plex Mouse/Rat FGF-21 Cartridge
There are currently no reviews for this product. Be the first to review Simple Plex Mouse/Rat FGF-21 Cartridge and earn rewards!
Have you used Simple Plex Mouse/Rat FGF-21 Cartridge?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review