Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
R&D Systems | Catalog # SPCKB-MP-004049
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Osteopontin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Osteopontin control were tested in multiple assays performed by at least three technicians using two lots of reagents for Osteopontin.
Cerebrospinal Fluid, Heparin Plasma, Mouse Cerebrospinal Fluid, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Mouse Urine, Rat EDTA Plasma, Rat Serum, Rat Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 19 |
| Mean (pg/mL) | 24.7 | 1229 | 24.2 | 1210 |
| Standard Deviation | 1.03 | 117 | 2.12 | 130 |
| CV% | 4.2 | 9.5 | 8.7 | 10.8 |
Recovery for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
Recovery for the mouse/rat Osteopontin assays was evaluated at three different spiked concentrations of Osteopontin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Urine (n=4) | 108 | 96-120 |
Linearity
Samples containing and/or spiked with high concentrations of mouse/rat Osteopontin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
Simple Plex Mouse/Rat Osteopontin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse/Rat Osteopontin Assay. Assay range is 1.20-4580 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Osteopontin/OPN
Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adÂhesion, and migration. Its expression is upregulatÂed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.
Long Name
Alternate Names
Gene Symbol
Additional Osteopontin/OPN Products
Product Documents for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge
For research use only
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