Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Catalog #: SPCKB-MP-004049 Datasheet / COA / SDS
Simple Plex Mouse/Rat Osteopontin/OPN assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
Control Products Available
Mouse/Rat OPN Ella Assay
4 Images
Product Details
Supplemental Products

Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Summary

Sample Type & Volume Required Per Well*
Mouse Serum (500 nL), Rat Serum (500 nL), Mouse EDTA Plasma (500 nL), Rat EDTA Plasma (500 nL), Mouse Heparin Plasma (500 nL), Mouse Urine (25 nL), Rat Urine (500 nL), Mouse Cerebrospinal Fluid (500 nL)
0.28 pg/mL
Assay Range
1.2 - 4,580 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid)
Natural and recombinant mouse/rat Osteopontin.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of mouse and rat Osteopontin in serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 19
Mean (pg/mL) 24.7 1229 24.2 1210
Standard Deviation 1.03 117 2.12 130
CV% 4.2 9.5 8.7 10.8


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 108 96-120


Samples containing and/or spiked with high concentrations of mouse/rat Osteopontin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Mouse OPN Ella Assay Linearity
Rat OPN Ella Assay Linearity

Scientific Data

Mouse/Rat OPN Ella Assay Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Long Name:
Secreted Phosphoprotein 1 [BNSP]
Entrez Gene IDs:
6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)
Alternate Names:
BNSP; Bone sialoprotein 1; Eta-1; MGC110940; Nephropontin; OPN; Osteopontin; secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1); secreted phosphoprotein-1 (osteopontin, bone sialoprotein); Spp1; SPP-1; SPP1/CALPHA1 fusion; Urinary stone protein; uropontin


No product specific FAQs exist for this product, however you may

View all Simple Plex Assays FAQs

Automated ELISA Instruments

Simple Plex Assay Controls

Reviews for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Mouse/Rat Osteopontin/OPN Cartridge and earn rewards!

Have you used Simple Plex Mouse/Rat Osteopontin/OPN Cartridge?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review