Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

R&D Systems | Catalog # SPCKB-MP-004049

Simple Plex Mouse/Rat Osteopontin/OPN assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer.
R&D Systems

Customize My Cartridge on Bio-Techne

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com

Customize My Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Mouse Serum (500 nL), Rat Serum (500 nL), Mouse EDTA Plasma (500 nL), Rat EDTA Plasma (500 nL), Mouse Heparin Plasma (500 nL), Mouse Urine (25 nL), Rat Urine (500 nL), Mouse Cerebrospinal Fluid (500 nL)

Sensitivity

0.28 pg/mL

Assay Range

1.2-4580 pg/mL (Mouse Serum, Rat Serum, Mouse EDTA Plasma, Rat EDTA Plasma, Heparin Plasma, Mouse Heparin Plasma, Mouse Urine, Rat Urine, Cerebrospinal Fluid, Mouse Cerebrospinal Fluid)

Simple Plex Mouse/Rat Osteopontin/OPN Cartridge Summary

Simple Plex assay for the detection of mouse and rat Osteopontin in serum, plasma (EDTA/Heparin), urine, and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Osteopontin/OPN Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Mouse, Rat

Specificity

Natural and recombinant mouse/rat Osteopontin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cerebrospinal Fluid, Heparin Plasma, Mouse Cerebrospinal Fluid, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum, Mouse Urine, Rat EDTA Plasma, Rat Serum, Rat Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 21 19
Mean (pg/mL) 24.7 1229 24.2 1210
Standard Deviation 1.03 117 2.12 130
CV% 4.2 9.5 8.7 10.8

Recovery for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 108 96-120

Linearity

Samples containing and/or spiked with high concentrations of mouse/rat Osteopontin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Mouse/Rat OPN Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteopontin/OPN

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad­hesion, and migration. Its expression is upregulat­ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

For research use only

Citations for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

View all citations for the Simple Plex platform on Bio-Techne.com.

Please visit our Instrument Citation Database on Bio-Techne.com to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Mouse/Rat Osteopontin/OPN Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Mouse/Rat Osteopontin/OPN Cartridge and earn rewards!

Have you used Simple Plex Mouse/Rat Osteopontin/OPN Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card